Identification of Gene Diversity of Melanocortin-4 Receptor and its Relationship with the Birth and Body Size of Beef Cattle

Melanocortin-4 Receptor (MC4R) expressed in hypothalamus which is part of the brain that are involved regulation appetite, metabolism and bodyweight. This study aimed to identify MC4R gene diversity second cross generation Belgian Blue, Wagyu, Brahman crossbred its relationship with body weight size birth. Polymerase Chain Reaction (PCR) DNA sequencing were used Single Nucleotide Polymorphism (SNP). The result this showed genes was polymorphic one SNP at 1133 C>G included missense mutation. has two allels C G three genotypes. Test Hardy Weinberg equilibrium population cattle equilibrium. heterozigosity values had a low genetics. association Genes not able indicate difference between weight, length, heart girth wither’s height Based on study, it can be concluded polymorphism only distinguish genotypes but cannot as selection tools for birth.
 Keywords: Crossbreed beef cattle, Diversity, Heterozigosity, gene, SNP.